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This study was intended to show that the amount of both serum albumin and hemoglobin has some influence in determining serum amylase by chromogenic method and amyloclastic method. 1. In chromogenic method, the influence of serum albumin shows the increase of 29.4±10.9% with amylochrome method. But in amyloclastic method, it shows as much as 6.1±5.6%. 2. With diluted albumin, the amylochrome method shows no difference below 1.0 gm/dl. 3. With hemoglobin below 600 mg/dl, the amylochrome method also shows no change. But in amyloclastic method, the measured values of serum amylase shows a high increase from the low concentration level, resulting in considerable test errors. 4. The correlation between amylochrome and amyloclastic method was remarkably fair. (n=61, r=0.997, y=1.002x+0.7) As a result, the chromogenic method includes fewer test errors than other methods such as amyloclastic method, and shows less difference in the presence of serum albumin or hemoglobin(hemolysis).
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